Why ‘rm -rf’ is scarier than a pipette tip, and other truths of digital biology. Introduction: Hello, World (of Omics)
Hi! I’m David. Ask me what I do, and you’ll get a different answer depending on the day. david bioinfo
I’ve learned the hard way that a single misplaced flag in cutadapt can turn your precious RNA-seq reads into biological confetti. My morning ritual? Coffee. htop to see if my server is crying. And grep to make sure my adapter indices didn’t cross-contaminate. Why ‘rm -rf’ is scarier than a pipette
I found 10,000 variants. The lab expected 5. Did I mis-call indels? Is there a batch effect? Did someone accidentally use the mouse reference genome again? (It happened once. Once.) david bioinfo